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In compiling this volume, we have attempted to collate both established and novel experimental methods that can be used to study this debilitating condition. The first edition of this book gathered together a collection of techniques that focused on solving the inherent problems that exist in renal research, most notably the complexity of renal anatomy and physiology and the associated plethora of different cell types found in the kidney.
It is hoped that the new volume complements the first edition by emphasizing recent innovations that are relevant to the questions currently being asked in basic renal research. Winter Glostrup massage addition the present volume takes a Fast hookups in Danmark holistic approach by considering associated disorders such as complications seen with peritoneal dialysis, the cardiorenal syndrome, and vascular calcification.
Part I of the second edition provides a number of in vitro, in vivo, and ex vivo models of kidney disease and associated complications. The first two chapters describe the isolation and propagation of podocytes Chapter 1 and fibroblasts Chapter 2while Chapters 3 and 4 address the Cj massage Stenlose ks of peritoneal dialysis complications.
An important technique for studying the poorly understood sympathetic nervous system link between renal and cardiac disease is provided in Chapter 5. In a number of organs, decellularized extracellular matrix is being used to provide both a scaffold for organ regeneration studies and a Cj massage Stenlose ks accurate ex vivo representation of the in vivo microenvironment.
Jin et al. Part II looks at recent advances in imaging techniques.
Estimation of glomerular num- ber has long been a tedious and time-consuming process, poorly suited to Stenlkse stud- ies with even small numbers of animals. The exciting developments described in Chapter 7 provide a means of measuring glomerular endowment through magnetic resonance imag- ing.
Likewise in Chapter 8 Ashraf et al. The next two parts provide protocols to study two important clinical complications experimentally.
Part III covers recent advances in studying metabolism in renal ischemia and reperfusion via telemetry Chapter 9magnetic resonance imaging Chapters 10 and 11Syenlose multiphoton microscopy Chapter Part IV addresses the study and measure- ment of vascular calcification, an important manifestation of mawsage disturbances in bone metabolism and mineral handling. This includes a state-of-the-art review massagd the topic Chapter 13in addition to protocols of an in vitro model of mineralization Chapter 14the isolation and quantitation of novel mediators of extraosseous mineral stress Chapter 15Cj massage Stenlose ks fluorescent imaging of vascular calcification Chapter Finally, the last three chapters Part V provide analytical techniques that are both topi- cal and of widespread relevance to the study of experimental renal disease.
These are improved methodologies for studying protein phosphorylation in signal transduction Chapter 17laser capture microdissection Chapter 18and Flong online sex situ insights into the epig- enome Chapter V VI Preface Nephrology is a discipline that combines both basic and clinical sciences.
In editing this k, we have attempted to provide a Male Randers stars naked of protocols useful to those with some laboratory experience in Nnephrology and those new to the field. Protocols are written in the standard Methods in C Biolojjy format which includes a general introduction, an extensive list of materials required, and stepwise instructions to follow.